
●Af1521 Macrodomain Covalent Affinity Resin (agarose)
Description: Cat. #2506 Af1521 Macrodomain Covalent Resin is highly purified Af1521 macrodomain fusion protein expressed in E. coli, and covalently bound to 4% cross-linked agarose (resin). Typical conditions for the elution of the affinity bound proteins to the resin, for example SDS-PAGE sample buffer, will not dissociate the Af1521 macrodomain affinity protein from the resin. Tulip Biolabs Cat. #2426 macrodomain beads, above, are similar except bound to magnetic beads. The resin is useful for affinity purification (pulldown) of MARylated and PARylated proteins.
See the Cat. #2507 negative control beads, below.
Supplied as: 0.5mg purified Af1521 macrodomain fusion protein covalently bound to 4% cross-linked agarose beads in 0.5 mL buffer.
Please see the Cat. #2506 Datasheet for a complete description.
Price: Cat. #2506 0.5mg/$325; 5 x 0.5mg/$1300
Contact us for custom packaging and larger quantities.
Available in a set, Cat. #4508

●Af1521 Macrodomain Negative Control Resin (agarose beads)
Description: Identical to the #2302 affinity resin except for two gly to asp substitutions in the macrodomain sequence, which abolishes ligand binding. This negative control resin is useful as a control for non-specific binding.
Supplied as: 0.5mg protein/0.5mL buffer as a suspension containing ~50-75µL resin.
Please see the Cat. #2303 Datasheet for a complete description.
Price: Cat. #2303 0.5mg/$125; 5 x 0.5mg/$500
Contact us for custom packaging and larger quantities.
Available in a set, Cat. #4301
●Negative Control Magnetic Resin (covalent)
Description: Cat. #2427 Negative Control Magnetic Resin are the same magnetic beads used to produce the #2414, 2426, and 2438 mag affinity resins, but instead of attaching the corresponding affinity protein to the resin, the resin is modified to an alpha amino carboxylic acid. Use equivalent volume of the 2427 neg control mag resin as experimentally used for the 2414, 2426, or 2438 mag affinity resins as a control for non-specific binding.
Supplied as: Each vial contains 0.5mL Negative Control magnetic resin beads in approximately equivalent quantity as the mag affinity resins 2414, 2426, and 2438.
Please see the Cat. #2427 Datasheet for a complete description.
Price: Cat. #2427 0.5mL/$150; 5 x 0.5mL/$600
Contact us for custom packaging and larger quantities.
●Af1521 Macrodomain Affinity Magnetic Resin (covalent)
Description: Cat. #2426 Af1521 Macrodomain Magnetic Resin is highly purified Af1521 macrodomain fusion protein expressed in E. coli, and covalently bound to superparamagnetic silica-based beads (resin). Typical conditions for the elution of the affinity bound proteins to the resin, for example SDS-PAGE sample buffer, will not dissociate the Af1521 macrodomain affinity protein from the resin. The magnetic resin beads are small, with an average 1µm diameter, which remain suspended in solution for a few minutes after mixing, allowing for convenient and accurate pipetting. Tulip Biolabs Cat. #2302 (non-magnetic) and #2305 (magnetic) macrodomain beads, above, are composed of the same affinity protein except non-covalently bound to glutathione beads. The resin is useful for affinity purification (pulldown) of MARylated and PARylated proteins.
See the Cat. #2427 negative control beads, below.
Supplied as: 0.5mg purified Af1521 macrodomain fusion protein covalently bound to 1µm superparamagnetic resin beads (orange dyed) in 0.5 mL buffer.
Please see the Cat. #2426 Datasheet for a complete description.
Price: Cat. #2426 0.5mg/$500; 5 x 0.5mg/$2000
Contact us for custom packaging and larger quantities.
●Af1521 Macrodomain Affinity Magnetic Resin
Description: Cat. #2305 is the same construct as Cat. #2302, above, except here it is supplied bound to super paramagnetic glutathione beads for easier handling and higher throughput.
Supplied as: 1mg protein/1mL buffer as a suspension containing ~300µL resin.
Please see the Datasheet for a complete description and ref AZ Lu et al (2019).
Price: Cat. #2305 1mg/$625; 5 x 1mg/$2500
Contact us for custom packaging and larger quantities.
Available in a set, Cat. #4319
●Af1521 Macrodomain Affinity Resin Set (agarose beads)
Supplied as: Set contains 1 each of the following (or can be ordered separately, above):
•Af1521 Macrodomain Affinity Resin, 1mg, Cat. #2302
•Af1521 Macrodomain Negative Control Resin, 0.5mg, Cat. #2303
Please see the Cat. #4301 Datasheet for a complete description.
Price: Cat. #4301 1 set/$575; 5 x 1 set/$2300
Contact us for custom packaging and larger quantities.
Af1521 Macrodomain (PAR/MAR) Affinity Resins


Our product areas > Poly/mono ADP-ribose Products > PAR/MAR Affinity Resins > Af1521 Macrodomain > NEXT
●Af1521 Macrodomain (Poly- and Mono-ADP-ribose) Affinity Resins
The Tulip Biolabs, Inc. Af1521 Macrodomain Affinity Resins are designed for the isolation of poly-ADP-ribosylated (PARylated) and mono-ADP-ribosylated (MARylated) proteins from cultured cells and tissue lysates.
Af1521 is a thermopohilic protein from Archaeoglobus fulgidus, and contains a conserved ~190 amino acid domain known as the macrodomain. Macrodomains are found in a wide variety of organisms including bacteria, viruses, and vertebrates. Expressed and purified macrodomains from Af1521, Alc1, macroH2A and Bal/PARP9 proteins have been shown to bind to a subset of polymeric ADP-ribose modified proteins with high specificity and affinity. The Af1521 macrodomain also binds to a subset of mono ADP-ribose modified proteins. Through the use of Af1521 macrodomain affinity resins, poly-ADP-ribosylated (PARylated) and mono-ADP-ribosylated (MARylated) proteins can be isolated from cell or tissue lysates. The resin bound proteins can be eluted from the affinity resin, and analyzed by immunoblotting, mass spec, and other methods. Tulip Biolabs produces four types of Af1521 macrodomain beads/resin, see below:
Cat. #2302 Af1521 GST-fusion protein bound to glut agarose beads
Cat. #2305 Af1521 GST-fusion protein bound to magnetic beads
Cat. #2426 Af1521 protein covalently bound to magnetic beads
●Af1521 Macrodomain Affinity Resin (agarose beads)
Description: Highly purified GST-Af1521 macrodomain fusion protein construct expressed in E. coli, and bound to glutathione agarose beads. The Af1521 macrodomain protein binds poly- and mono-ADP-ribosylated proteins, plus free ADP-ribose. See the Cat. #2303 negative control beads, below.
Supplied as: 1mg protein/1mL buffer as a suspension containing ~100µL resin.
Please see the Cat. #2302 Datasheet for a complete description.
Price: Cat. #2302 1mg/$450; 5 x 1mg/$1800
Contact us for custom packaging and larger quantities.
Available in a set, Cat. #4301
●Negative Control Covalent Resin (agarose)
Description: Cat. #2507 Negative Control Covalent Resin are the same agarose beads used to produce the #2506 macrodomain affinity resin, but instead of attaching the corresponding affinity protein to the resin, the resin is modified to an alpha amino carboxylic acid. Use equivalent volume of the 2507 neg control covalent resin as experimentally used for the 2506, covalent affinity resin as a control for non-specific binding. Each vial contains 0.5mL Negative Control Covalent Resin in approximately equivalent quantity as the #2506 resin.
Please see the Cat. #2507 Datasheet for a complete description.
Price: Cat. #2507 0.5mL/$100; 5 x 0.5mL/$400
Contact us for custom packaging and larger quantities.
●Af1521 Macrodomain Covalent Resin Set (agarose)
Supplied as: Set contains 1 each of the following (or can be ordered separately, above):
•Af1521 Macrodomain Covalent Affinity Resin, 0.5mg, Cat. #2506
•Af1521 Negative Control Covalent Resin, 0.5mL, Cat. #2507
Please see the Cat. #4508 Datasheet for a complete description.
Price: Cat. #4508 1 set/$425; 5 x 1 set/$1700
Contact us for custom packaging and larger quantities.
Af1521 Macrodomain Affinity Resins are available in multiple formats.
Contact us for custom packaging and synthesis!
●Af1521 Macrodomain Affinity Mag Resin Set
Supplied as: Set contains 1 each of the following (or can be ordered separately, above):
•Af1521 Macrodomain Affinity Mag Resin, 1mg, Cat. #2305
•Af1521 Negative Control Mag Resin, 0.5mg, Cat. #2306
Please see the Datasheet for a complete description.
Price: Cat. #4319 1 set/$775; 5 x 1 set/$3100
Contact us for custom packaging and larger quantities.
●Af1521 Negative Control Mag Resin
Description: Identical to the #2305 affinity resin except for two gly to asp substitutions in the macrodomain sequence, which abolishes ligand binding. This negative control mag resin is useful as a control for non-specific binding of #2305.
Supplied as: 0.5mg protein/0.5mL buffer as a suspension containing ~150µL resin.
Please see the Datasheet for a complete description.
Price: Cat. #2306 0.5mg/$150; 5 x 0.5mg/$600
Contact us for custom packaging and larger quantities.
Available in a set, Cat. #4319

